Transduction with Integration-Defective Mutants of Salmonella typhimurium Bacteriophage KB1

Transduction by bacteriophage P22 in nonsmooth mutants of Salmonella typhimurium.

The general transducing phage P22 attacks only smooth (S) Salmonella with O antigen 12, determined by the oligosaccharide repeating unit constituting the distal part of the somatic lipopolysaccharide (LPS) side chain; non-S mutants, whose LPS contain few or no O repeating units, appear to be resistant. Auxotrophic non-S mutants of Salmonella typhimurium LT2 were tested as transductional recipie...

Generalized transduction by bacteriophage P22 in Salmonella typhimurium. II. Mechanism of integration of transducing DNA.

Generalized transduction by bacteriophage P22 occurs by the transfer of DNA from one bacterium to another by means of a phage-like particle (i.e. a transducing particle) which contains bacterial DNA formed before infection (preceding paper). Transduction of a 3aP-labeled, light recipient with purified transducing particles whose DNA is labeled with 3H, zH, 16N, 13C results in the physical assoc...

Salmonella typhimurium mutants defective in cytidine monophosphate kinase (cmk).

Mutants of Salmonella typhimurium defective in cytidine 5'-monophosphate (CMP) kinase (cmk) have been isolated. The mutants also lack the ability to phosphorylate 2'-deoxyCMP, indicating that one enzyme is responsible for the phosphorylation of both CMP and deoxyCMP to the corresponding diphosphates. In glucose minimal medium the mutants grow at the same rate as the parental strain; however, th...

Salmonella typhimurium prfA mutants defective in release factor 1.

Mutations have been characterized that map in the prfA gene of Salmonella typhimurium. These weak amber suppressors show increased readthrough of UAG but not UAA or UGA codons. Some hemA mutants exhibit a similar suppressor activity due to transcriptional polarity on prfA. All of the suppressors mapping in prfA are recessive to the wild type. Two mutant prfA genes were cloned onto plasmids, and...

Salmonella typhimurium proline mutants.